Search results for " protein aggregation"
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
The mechanism of protein aggregation is not well understood, “but most proteins will aggregate when they are in an unfolded or partially folded state,” says Angelica Olcott, product manager at Protein…
Article
Predicting Progress in Protein Aggregation
External factors do not alone determine when protein aggregation will occur. The physicochemical characteristics of the short polypeptide segments that comprise protein therapeutics also determine the…
Article
Challenges of Protein Aggregation During Purification
In addition, protein aggregation can refer to a broad range of molecular behaviors, from dimer formation to visible particulates due to noncovalent interactions mediated by hydrophobicity and covalent…
Article
What If You Could Remove Your Antibody Aggregates Already in the Protein A Step?
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In this study, the potential of Protein A chromatography to separate antibody monomer from HMW species was investigated. A design of experiments approach…
Article
Developing a HIC polishing step for removal of mAb aggregates
This article describes the development of a flow-through (FT) polishing step using hydrophobic interaction chromatography (HIC) to remove mAb aggregates.
It includes:
- The use of HTPD plate…
Article
Modeling the Degradation of mAb Therapeutics
Mathematical modeling of protein aggregation correlated along with kinetic data can help developers gain both qualitative and quantitative insights into the mechanism behind aggregation. Armed with th…
Article
Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
The freezing rate in a single freeze-thaw cycle had negligible impact on protein aggregation when fast-thawing conditions were used to thaw. Slow thawing led to higher protein aggregation and subvisib…
Article
Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015
By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography ha…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Newer classes of biotherapies will require innovations in processing technology.
By Glen R. Bolton, Bernard N. Violand, Richard S. Wright, Shujun Sun, Khurram M. Sunasara, Kathleen Watson, Johna…
Article
Optimization of Protein Expression in Escherichia Coli
May 01, 2015
By Siavash Bashiri, David Vikström, Nurzian Ismail
BioPharm International
Volume 28, Issue 5
Production of proteins for manufacturing therapeutics and pharmaceuticals is a c…